Metabolites, Liver Disease, Library Match

DOCUMENT: Application | Ref: 203-821-422Download
Category: Metabolomics, Separation Science Mass Spectrometry, TOF Mass Spectrometry

Application of GC High Resolution Time-of-Flight Mass Spectrometry to Mouse Liver Metabolomic Analyses

We demonstrate here that the Pegasus GC-HRT provides a tool for the analysis of metabolic changes in extracts of normal and diseased liver. Three primary metabolites— isoleucine, leucine, and octadecenoic acid—are identified based on library matches to acquired spectra and accurate mass matches of molecular and fragment ions. It proves a powerful tool in the analysis of metabolic changes due, in this instance, to PCBs and ethanol.

Keywords: metabolomics, profiling, alcoholic liver disease, fatty acid metabolism, GCMS